Christendat Lab

Cell and Systems Biology, University of Toronto


For a comprehensive list of published work, refer to the pubmed link below :

Updated Publications

Selected Publications:


Structural and biochemical approaches uncover multiple evolutionary trajectories of plant quinate dehydrogenases.. Gritsunov A, Peek J, Diaz Caballero J, Guttman D, Christendat D. Plant J. 2018 June 11.

Shikimate Induced Transcriptional Activation of Protocatechuate Biosynthesis Genes by QuiR, a LysR-Type Transcriptional Regulator, in Listeria monocytogenes. Prezioso SM, Xue K, Leung N, Gray-Owen SD, Christendat D. J Mol Biol. 2018 Apr 27;430(9):1265-1283.


Structurally diverse dehydroshikimate dehydratase variants participate in microbial quinate catabolism.Peek J, Roman J, Moran GR, Christendat D. Mol Microbiol. 2017 Jan;103(1):39-54.


The shikimate dehydrogenase family: functional diversity within a conserved structural and mechanistic framework. Peek J, Christendat D. Arch Biochem Biophys. 2015 Jan 15;566:85-99.


Isolation and molecular characterization of the shikimate dehydrogenase domain from the Toxoplasma gondii AROM complex. Peek J, Castiglione G, Shi T, Christendat D.Mol. Biochem. Parasitol. 2014 Mar-Apr;194(1-2):16-9

Identification of Novel Polyphenolic Inhibitors of Shikimate Dehydrogenase (AroE). Peek J, Shi T, Christendat D.J Biomol Screen 2014 Mar;19(7):1090-1098


Crystal structure and biochemical analyses reveal that the Arabidopsis triphosphate tunnel metalloenzyme AtTTM3 is a tripolyphosphatase involved in root development.Moeder W, Garcia-Petit C, Ung H, Fucile G, Samuel MA, Christendat D, Yoshioka K.Plant J. 2013 Nov;76(4):615-26

Sequencing and annotation of the Ophiostoma ulmi genome.Khoshraftar S, Hung S, Khan S, Gong Y, Tyagi V, Parkinson J, Sain M, Moses AM, Christendat D.BMC Genomics 2013;14:162

Insights into the function of RifI2: structural and biochemical investigation of a new shikimate dehydrogenase family protein.Peek J, Garcia C, Lee J, Christendat D.Biochim. Biophys. Acta 2013 Feb;1834(2):516-23


Structural and mechanistic analysis of a novel class of shikimate dehydrogenases: evidence for a conserved catalytic mechanism in the shikimate dehydrogenase family.Peek J, Lee J, Hu S, Senisterra G, Christendat D.Biochemistry 2011 Oct;50(40):8616-27

Structural and biochemical investigation of two Arabidopsis shikimate kinases: the heat-inducible isoform is thermostable.Fucile G, Garcia C, Carlsson J, Sunnerhagen M, Christendat D.Protein Sci. 2011 Jul;20(7):1125-36

ePlant and the 3D data display initiative: integrative systems biology on the world wide web.Fucile G, Di Biase D, Nahal H, La G, Khodabandeh S, Chen Y, Easley K, Christendat D, Kelley L, Provart NJ.PLoS ONE 2011;6(1):e15237


The crystal structure of Aquifex aeolicus prephenate dehydrogenase reveals the mode of tyrosine inhibition.Sun W, Shahinas D, Bonvin J, Hou W, Kimber MS, Turnbull J, Christendat D.J. Biol. Chem. 2009 May;284(19):13223-32


Evolutionary diversification of plant shikimate kinase gene duplicates.Fucile G, Falconer S, Christendat D.PLoS Genet. 2008 Dec;4(12):e1000292

A phylogenomic analysis of the shikimate dehydrogenases reveals broadscale functional diversification and identifies one functionally distinct subclass.Singh S, Stavrinides J, Christendat D, Guttman DS.Mol. Biol. Evol. 2008 Oct;25(10):2221-32

dentification of a functionally essential amino acid for Arabidopsis cyclic nucleotide gated ion channels using the chimeric AtCNGC11/12 gene.Baxter J, Moeder W, Urquhart W, Shahinas D, Chin K, Christendat D, Kang HG, Angelova M, Kato N, Yoshioka K.Plant J. 2008 Nov;56(3):457-69

Structural insight on the mechanism of regulation of the MarR family of proteins: high-resolution crystal structure of a transcriptional repressor from Methanobacterium thermoautotrophicum.Saridakis V, Shahinas D, Xu X, Christendat D.J. Mol. Biol. 2008 Mar;377(3):655-67


Structure of Arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway.Singh SA, Christendat D.Biochemistry 2006 Jun;45(25):7787-96

Biochemical characterization of prephenate dehydrogenase from the hyperthermophilic bacterium Aquifex aeolicus.Bonvin J, Aponte RA, Marcantonio M, Singh S, Christendat D, Turnbull JL.Protein Sci. 2006 Jun;15(6):1417-32

Crystal structure of prephenate dehydrogenase from Aquifex aeolicus. Insights into the catalytic mechanism.Sun W, Singh S, Zhang R, Turnbull JL, Christendat D.J. Biol. Chem. 2006 May;281(18):12919-28


The crystal structure of a novel SAM-dependent methyltransferase PH1915 from Pyrococcus horikoshii.Sun W, Xu X, Pavlova M, Edwards AM, Joachimiak A, Savchenko A, Christendat D.Protein Sci. 2005 Dec;14(12):3121-8

Crystal structure of a novel shikimate dehydrogenase from Haemophilus influenzae.Singh S, Korolev S, Koroleva O, Zarembinski T, Collart F, Joachimiak A, Christendat D.J. Biol. Chem. 2005 Apr;280(17):17101-8


Crystal structure of the hypothetical protein TA1238 from Thermoplasma acidophilum: a new type of helical super-bundle.Sanishvili R, Pennycooke M, Gu J, Xu X, Joachimiak A, Edwards AM, Christendat D.J. Struct. Funct. Genomics 2004;5(4):231-40

The structural basis for methylmalonic aciduria. The crystal structure of archaeal ATP:cobalamin adenosyltransferase.Saridakis V, Yakunin A, Xu X, Anandakumar P, Pennycooke M, Gu J, Cheung F, Lew JM, Sanishvili R, Joachimiak A, Arrowsmith CH, Christendat D, Edwards AM.J. Biol. Chem. 2004 May;279(22):23646-53

Crystal structure of chorismate synthase from Aquifex aeolicus reveals a novel beta alpha beta sandwich topology.Viola CM, Saridakis V, Christendat D.Proteins 2004 Jan;54(1):166-9

Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum.Kim Y, Yakunin AF, Kuznetsova E, Xu X, Pennycooke M, Gu J, Cheung F, Proudfoot M, Arrowsmith CH, Joachimiak A, Edwards AM, Christendat D.J. Biol. Chem. 2004 Jan;279(1):517-26


Crystal structures of MTH1187 and its yeast ortholog YBL001c.Tao X, Khayat R, Christendat D, Savchenko A, Xu X, Goldsmith-Fischman S, Honig B, Edwards A, Arrowsmith CH, Tong L.Proteins 2003 Aug;52(3):478-80

Data mining crystallization databases: knowledge-based approaches to optimize protein crystal screens.Kimber MS, Vallee F, Houston S, Necakov A, Skarina T, Evdokimova E, Beasley S, Christendat D, Savchenko A, Arrowsmith CH, Vedadi M, Gerstein M, Edwards AM.Proteins 2003 Jun;51(4):562-8

Structural proteomics: toward high-throughput structural biology as a tool in functional genomics.Yee A, Pardee K, Christendat D, Savchenko A, Edwards AM, Arrowsmith CH.Acc. Chem. Res. 2003 Mar;36(3):183-9

Deep trefoil knot implicated in RNA binding found in an archaebacterial protein.Zarembinski TI, Kim Y, Peterson K, Christendat D, Dharamsi A, Arrowsmith CH, Edwards AM, Joachimiak A.Proteins 2003 Feb;50(2):177-83


The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase.Keller JP, Smith PM, Benach J, Christendat D, deTitta GT, Hunt JF.Structure 2002 Nov;10(11):1475-87

Crystal structure of MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase.Batra R, Christendat D, Edwards A, Arrowsmith C, Tong L.Proteins 2002 Nov;49(2):285-8

The crystal structure of hypothetical protein MTH1491 from Methanobacterium thermoautotrophicum.Christendat D, Saridakis V, Kim Y, Kumar PA, Xu X, Semesi A, Joachimiak A, Arrowsmith CH, Edwards AM.Protein Sci. 2002 Jun;11(6):1409-14

Crystal structure of Methanobacterium thermoautotrophicum conserved protein MTH1020 reveals an NTN-hydrolase fold.Saridakis V, Christendat D, Thygesen A, Arrowsmith CH, Edwards AM, Pai EF.Proteins 2002 Jul;48(1):141-3

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